crispr cas9 system (Santa Cruz Biotechnology)
94
Structured Review
Santa Cruz Biotechnology
crispr cas9 system
Crispr Cas9 System, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispr+cas9+plasmids/Heme+Oxygenase+1+CRISPR%2FCas9+KO+Plasmid/pm41934679-54-4-12
Average 94 stars, based on 4 article reviews
Crispr Cas9 System, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crispr+cas9+plasmids/Heme+Oxygenase+1+CRISPR%2FCas9+KO+Plasmid/pm41934679-54-4-12
Average 94 stars, based on 4 article reviews
crispr cas9 system - by Bioz Stars,
2026-09
94/100 stars
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CRISPR:Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis Article Snippet: .. Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ Article Snippet: The AGS cell line with the cortactin gene knockout was generated previously using the CRISPR/Cas9 plasmids (#sc-400761, Santa Cruz Biotechnology, Heidelberg, Germany) and the HDR-plasmid (#sc-400761-HDR, Santa Cruz Biotechnology) as described (Knorr et al. ). .. The AGS cell line with fak gene knockout was generated using the Article Title: Mitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolism Article Snippet: ACP cDNA (OriGene, RC208941) was subcloned into a pcDNA 5/FRT/TO with a C-terminal FLAG-tag. .. The Article Title: CD4 + T-cells sensitize quasi-mesenchymal breast tumors lacking CD73 to anti-CTLA4 immune checkpoint blockade therapy Article Snippet: .. In order to generate the CD73 and B2M double knock out (DKO) cell line, B2M was knocked out from the sgCD73 murine cell line via transient transfection using Article Title: Hexokinase-I directly binds to a charged membrane-buried glutamate of mitochondrial VDAC1 and VDAC2. Article Snippet: .. Generation of HKI-KO and VDAC-DKO cell lines To knock out HKI in HeLa cells, we obtained a mix of three different Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis. Article Snippet: First release: 8 January 2026 science.org (Page numbers not final at time of first release) 1 Eukaryotic cells possess an inherent ability to switch fuel sources in response to nutrient availability and physiological demands.. This metabolic flexibility enables cells and tissues to utilize distinct pools of substrates, such as carbohydrates, lipids, and amino acids, in response to their energetic and biosynthetic needs (1, 2).. This flexibility is particularly important during adaptation to fasting and cold exposure. Article Title: SLC25A48 controls mitochondrial choline import and metabolism Article Snippet: All constructs were confirmed by sequencing. pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). .. Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ Article Snippet: These AGS cells were grown at 37°C and 5% CO 2 in RPMI 1640 medium (Gibco, Darmstadt, Germany) supplemented with 10% fetal calf serum (Gibco, Paisley, UK), 1% antibiotic mix penicillin/streptomycin (Sigma-Aldrich, Steinheim, Germany), and 0.2% Normocin® (InvivoGen, Toulouse, France). .. The AGS cell line with the cortactin gene knockout was generated previously using the Control:Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis Article Snippet: .. Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis. Article Snippet: First release: 8 January 2026 science.org (Page numbers not final at time of first release) 1 Eukaryotic cells possess an inherent ability to switch fuel sources in response to nutrient availability and physiological demands.. This metabolic flexibility enables cells and tissues to utilize distinct pools of substrates, such as carbohydrates, lipids, and amino acids, in response to their energetic and biosynthetic needs (1, 2).. This flexibility is particularly important during adaptation to fasting and cold exposure. Article Title: SLC25A48 controls mitochondrial choline import and metabolism Article Snippet: All constructs were confirmed by sequencing. pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). .. Transfection:Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis Article Snippet: .. Article Title: CD4 + T-cells sensitize quasi-mesenchymal breast tumors lacking CD73 to anti-CTLA4 immune checkpoint blockade therapy Article Snippet: .. In order to generate the CD73 and B2M double knock out (DKO) cell line, B2M was knocked out from the sgCD73 murine cell line via transient transfection using Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis. Article Snippet: First release: 8 January 2026 science.org (Page numbers not final at time of first release) 1 Eukaryotic cells possess an inherent ability to switch fuel sources in response to nutrient availability and physiological demands.. This metabolic flexibility enables cells and tissues to utilize distinct pools of substrates, such as carbohydrates, lipids, and amino acids, in response to their energetic and biosynthetic needs (1, 2).. This flexibility is particularly important during adaptation to fasting and cold exposure. Article Title: SLC25A48 controls mitochondrial choline import and metabolism Article Snippet: All constructs were confirmed by sequencing. pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). .. Plasmid Preparation:Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis Article Snippet: .. Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis. Article Snippet: First release: 8 January 2026 science.org (Page numbers not final at time of first release) 1 Eukaryotic cells possess an inherent ability to switch fuel sources in response to nutrient availability and physiological demands.. This metabolic flexibility enables cells and tissues to utilize distinct pools of substrates, such as carbohydrates, lipids, and amino acids, in response to their energetic and biosynthetic needs (1, 2).. This flexibility is particularly important during adaptation to fasting and cold exposure. Article Title: SLC25A48 controls mitochondrial choline import and metabolism Article Snippet: All constructs were confirmed by sequencing. pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). .. Gene Knockout:Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ Article Snippet: The AGS cell line with the cortactin gene knockout was generated previously using the CRISPR/Cas9 plasmids (#sc-400761, Santa Cruz Biotechnology, Heidelberg, Germany) and the HDR-plasmid (#sc-400761-HDR, Santa Cruz Biotechnology) as described (Knorr et al. ). .. The AGS cell line with fak gene knockout was generated using the Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ Article Snippet: These AGS cells were grown at 37°C and 5% CO 2 in RPMI 1640 medium (Gibco, Darmstadt, Germany) supplemented with 10% fetal calf serum (Gibco, Paisley, UK), 1% antibiotic mix penicillin/streptomycin (Sigma-Aldrich, Steinheim, Germany), and 0.2% Normocin® (InvivoGen, Toulouse, France). .. The AGS cell line with the cortactin gene knockout was generated previously using the Generated:Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ Article Snippet: The AGS cell line with the cortactin gene knockout was generated previously using the CRISPR/Cas9 plasmids (#sc-400761, Santa Cruz Biotechnology, Heidelberg, Germany) and the HDR-plasmid (#sc-400761-HDR, Santa Cruz Biotechnology) as described (Knorr et al. ). .. The AGS cell line with fak gene knockout was generated using the Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ Article Snippet: These AGS cells were grown at 37°C and 5% CO 2 in RPMI 1640 medium (Gibco, Darmstadt, Germany) supplemented with 10% fetal calf serum (Gibco, Paisley, UK), 1% antibiotic mix penicillin/streptomycin (Sigma-Aldrich, Steinheim, Germany), and 0.2% Normocin® (InvivoGen, Toulouse, France). .. The AGS cell line with the cortactin gene knockout was generated previously using the Knock-Out:Article Title: CD4 + T-cells sensitize quasi-mesenchymal breast tumors lacking CD73 to anti-CTLA4 immune checkpoint blockade therapy Article Snippet: .. In order to generate the CD73 and B2M double knock out (DKO) cell line, B2M was knocked out from the sgCD73 murine cell line via transient transfection using Article Title: Hexokinase-I directly binds to a charged membrane-buried glutamate of mitochondrial VDAC1 and VDAC2. Article Snippet: .. Generation of HKI-KO and VDAC-DKO cell lines To knock out HKI in HeLa cells, we obtained a mix of three different |