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crispr cas9 system  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology crispr cas9 system
    Crispr Cas9 System, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+cas9+plasmids/Heme+Oxygenase+1+CRISPR%2FCas9+KO+Plasmid/pm41934679-54-4-12
    Average 94 stars, based on 4 article reviews
    crispr cas9 system - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    CRISPR:

    Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis
    Article Snippet: .. CRISPR/Cas9 plasmids for control and SLC25A45 KO in 293 cells (Santa Cruz Biotechnology; sc-418922, sc-410404, sc-410404-HDR) were transfected with UltraCruz transfection reagent and plasmid transfection medium (Santa Cruz Biotechnology; sc-395739, sc-108062). ..

    Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ
    Article Snippet: The AGS cell line with the cortactin gene knockout was generated previously using the CRISPR/Cas9 plasmids (#sc-400761, Santa Cruz Biotechnology, Heidelberg, Germany) and the HDR-plasmid (#sc-400761-HDR, Santa Cruz Biotechnology) as described (Knorr et al. ). .. The AGS cell line with fak gene knockout was generated using the CRISPR/Cas9 plasmids (#sc-400089-KO-2, Santa Cruz Biotechnology) and the HDR-plasmid (#sc-400089-HDR2, Santa Cruz Biotechnology). ..

    Article Title: Mitochondrial NADPH fuels mitochondrial fatty acid synthesis and lipoylation to power oxidative metabolism
    Article Snippet: ACP cDNA (OriGene, RC208941) was subcloned into a pcDNA 5/FRT/TO with a C-terminal FLAG-tag. .. The CRISPR–Cas9 plasmids were as follows: NADK2 (sc-414406) and IDH2 (sc-401417) The CRISPR–Cas9 plasmids were purchased from Santa Cruz. .. The guide RNAs targeting NNT, GLUD1 or MECR were cloned into a green fluorescent protein-expressing PX458 CRISPR vector (Addgene, 48138), and the sequences of each guide RNA are provided in Supplementary Table 1 .

    Article Title: CD4 + T-cells sensitize quasi-mesenchymal breast tumors lacking CD73 to anti-CTLA4 immune checkpoint blockade therapy
    Article Snippet: .. In order to generate the CD73 and B2M double knock out (DKO) cell line, B2M was knocked out from the sgCD73 murine cell line via transient transfection using CRISPR/Cas9 plasmids obtained from Santa Cruz Biotechnology. .. In order to generate the CD73 and B2M double knock out (DKO) cell line, B2M was knocked out from the sgCD73 murine cell line via transient transfection using CRISPR/Cas9 plasmids obtained from Santa Cruz Biotechnology.

    Article Title: Hexokinase-I directly binds to a charged membrane-buried glutamate of mitochondrial VDAC1 and VDAC2.
    Article Snippet: .. Generation of HKI-KO and VDAC-DKO cell lines To knock out HKI in HeLa cells, we obtained a mix of three different CRISPR/Cas9 plasmids for the corresponding gene from Santa Cruz Biotechnology (sc-401753-KO-2). ..

    Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis.
    Article Snippet: First release: 8 January 2026 science.org (Page numbers not final at time of first release) 1 Eukaryotic cells possess an inherent ability to switch fuel sources in response to nutrient availability and physiological demands.. This metabolic flexibility enables cells and tissues to utilize distinct pools of substrates, such as carbohydrates, lipids, and amino acids, in response to their energetic and biosynthetic needs (1, 2).. This flexibility is particularly important during adaptation to fasting and cold exposure.

    Article Title: SLC25A48 controls mitochondrial choline import and metabolism
    Article Snippet: All constructs were confirmed by sequencing. pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). .. CRISPR/Cas9 plasmids for control and SLC25A48-KO (Santa Cruz Biotechnology; sc-418922, sc-414730, sc-414730-HDR) were transfected with UltraCruz transfection reagent and plasmid transfection medium (Santa Cruz Biotechnology; sc-395739, sc-108062). ..

    Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ
    Article Snippet: These AGS cells were grown at 37°C and 5% CO 2 in RPMI 1640 medium (Gibco, Darmstadt, Germany) supplemented with 10% fetal calf serum (Gibco, Paisley, UK), 1% antibiotic mix penicillin/streptomycin (Sigma-Aldrich, Steinheim, Germany), and 0.2% Normocin® (InvivoGen, Toulouse, France). .. The AGS cell line with the cortactin gene knockout was generated previously using the CRISPR/Cas9 plasmids (#sc-400761, Santa Cruz Biotechnology, Heidelberg, Germany) and the HDR-plasmid (#sc-400761-HDR, Santa Cruz Biotechnology) as described (Knorr et al. ). .. The AGS cell line with fak gene knockout was generated using the CRISPR/Cas9 plasmids (#sc-400089-KO-2, Santa Cruz Biotechnology) and the HDR-plasmid (#sc-400089-HDR2, Santa Cruz Biotechnology).

    Control:

    Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis
    Article Snippet: .. CRISPR/Cas9 plasmids for control and SLC25A45 KO in 293 cells (Santa Cruz Biotechnology; sc-418922, sc-410404, sc-410404-HDR) were transfected with UltraCruz transfection reagent and plasmid transfection medium (Santa Cruz Biotechnology; sc-395739, sc-108062). ..

    Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis.
    Article Snippet: First release: 8 January 2026 science.org (Page numbers not final at time of first release) 1 Eukaryotic cells possess an inherent ability to switch fuel sources in response to nutrient availability and physiological demands.. This metabolic flexibility enables cells and tissues to utilize distinct pools of substrates, such as carbohydrates, lipids, and amino acids, in response to their energetic and biosynthetic needs (1, 2).. This flexibility is particularly important during adaptation to fasting and cold exposure.

    Article Title: SLC25A48 controls mitochondrial choline import and metabolism
    Article Snippet: All constructs were confirmed by sequencing. pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). .. CRISPR/Cas9 plasmids for control and SLC25A48-KO (Santa Cruz Biotechnology; sc-418922, sc-414730, sc-414730-HDR) were transfected with UltraCruz transfection reagent and plasmid transfection medium (Santa Cruz Biotechnology; sc-395739, sc-108062). ..

    Transfection:

    Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis
    Article Snippet: .. CRISPR/Cas9 plasmids for control and SLC25A45 KO in 293 cells (Santa Cruz Biotechnology; sc-418922, sc-410404, sc-410404-HDR) were transfected with UltraCruz transfection reagent and plasmid transfection medium (Santa Cruz Biotechnology; sc-395739, sc-108062). ..

    Article Title: CD4 + T-cells sensitize quasi-mesenchymal breast tumors lacking CD73 to anti-CTLA4 immune checkpoint blockade therapy
    Article Snippet: .. In order to generate the CD73 and B2M double knock out (DKO) cell line, B2M was knocked out from the sgCD73 murine cell line via transient transfection using CRISPR/Cas9 plasmids obtained from Santa Cruz Biotechnology. .. In order to generate the CD73 and B2M double knock out (DKO) cell line, B2M was knocked out from the sgCD73 murine cell line via transient transfection using CRISPR/Cas9 plasmids obtained from Santa Cruz Biotechnology.

    Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis.
    Article Snippet: First release: 8 January 2026 science.org (Page numbers not final at time of first release) 1 Eukaryotic cells possess an inherent ability to switch fuel sources in response to nutrient availability and physiological demands.. This metabolic flexibility enables cells and tissues to utilize distinct pools of substrates, such as carbohydrates, lipids, and amino acids, in response to their energetic and biosynthetic needs (1, 2).. This flexibility is particularly important during adaptation to fasting and cold exposure.

    Article Title: SLC25A48 controls mitochondrial choline import and metabolism
    Article Snippet: All constructs were confirmed by sequencing. pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). .. CRISPR/Cas9 plasmids for control and SLC25A48-KO (Santa Cruz Biotechnology; sc-418922, sc-414730, sc-414730-HDR) were transfected with UltraCruz transfection reagent and plasmid transfection medium (Santa Cruz Biotechnology; sc-395739, sc-108062). ..

    Plasmid Preparation:

    Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis
    Article Snippet: .. CRISPR/Cas9 plasmids for control and SLC25A45 KO in 293 cells (Santa Cruz Biotechnology; sc-418922, sc-410404, sc-410404-HDR) were transfected with UltraCruz transfection reagent and plasmid transfection medium (Santa Cruz Biotechnology; sc-395739, sc-108062). ..

    Article Title: Mitochondrial control of fuel switching via carnitine biosynthesis.
    Article Snippet: First release: 8 January 2026 science.org (Page numbers not final at time of first release) 1 Eukaryotic cells possess an inherent ability to switch fuel sources in response to nutrient availability and physiological demands.. This metabolic flexibility enables cells and tissues to utilize distinct pools of substrates, such as carbohydrates, lipids, and amino acids, in response to their energetic and biosynthetic needs (1, 2).. This flexibility is particularly important during adaptation to fasting and cold exposure.

    Article Title: SLC25A48 controls mitochondrial choline import and metabolism
    Article Snippet: All constructs were confirmed by sequencing. pMXs-3XHA-EGFP-OMP25 was obtained for MITO-Tag experiments (Addgene #83356). .. CRISPR/Cas9 plasmids for control and SLC25A48-KO (Santa Cruz Biotechnology; sc-418922, sc-414730, sc-414730-HDR) were transfected with UltraCruz transfection reagent and plasmid transfection medium (Santa Cruz Biotechnology; sc-395739, sc-108062). ..

    Gene Knockout:

    Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ
    Article Snippet: The AGS cell line with the cortactin gene knockout was generated previously using the CRISPR/Cas9 plasmids (#sc-400761, Santa Cruz Biotechnology, Heidelberg, Germany) and the HDR-plasmid (#sc-400761-HDR, Santa Cruz Biotechnology) as described (Knorr et al. ). .. The AGS cell line with fak gene knockout was generated using the CRISPR/Cas9 plasmids (#sc-400089-KO-2, Santa Cruz Biotechnology) and the HDR-plasmid (#sc-400089-HDR2, Santa Cruz Biotechnology). ..

    Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ
    Article Snippet: These AGS cells were grown at 37°C and 5% CO 2 in RPMI 1640 medium (Gibco, Darmstadt, Germany) supplemented with 10% fetal calf serum (Gibco, Paisley, UK), 1% antibiotic mix penicillin/streptomycin (Sigma-Aldrich, Steinheim, Germany), and 0.2% Normocin® (InvivoGen, Toulouse, France). .. The AGS cell line with the cortactin gene knockout was generated previously using the CRISPR/Cas9 plasmids (#sc-400761, Santa Cruz Biotechnology, Heidelberg, Germany) and the HDR-plasmid (#sc-400761-HDR, Santa Cruz Biotechnology) as described (Knorr et al. ). .. The AGS cell line with fak gene knockout was generated using the CRISPR/Cas9 plasmids (#sc-400089-KO-2, Santa Cruz Biotechnology) and the HDR-plasmid (#sc-400089-HDR2, Santa Cruz Biotechnology).

    Generated:

    Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ
    Article Snippet: The AGS cell line with the cortactin gene knockout was generated previously using the CRISPR/Cas9 plasmids (#sc-400761, Santa Cruz Biotechnology, Heidelberg, Germany) and the HDR-plasmid (#sc-400761-HDR, Santa Cruz Biotechnology) as described (Knorr et al. ). .. The AGS cell line with fak gene knockout was generated using the CRISPR/Cas9 plasmids (#sc-400089-KO-2, Santa Cruz Biotechnology) and the HDR-plasmid (#sc-400089-HDR2, Santa Cruz Biotechnology). ..

    Article Title: Enhanced ADP-heptose-dependent NF-κB activation by Helicobacter pylori CagA through cortactin-Src-dependent tyrosine phosphorylation of IKKβ
    Article Snippet: These AGS cells were grown at 37°C and 5% CO 2 in RPMI 1640 medium (Gibco, Darmstadt, Germany) supplemented with 10% fetal calf serum (Gibco, Paisley, UK), 1% antibiotic mix penicillin/streptomycin (Sigma-Aldrich, Steinheim, Germany), and 0.2% Normocin® (InvivoGen, Toulouse, France). .. The AGS cell line with the cortactin gene knockout was generated previously using the CRISPR/Cas9 plasmids (#sc-400761, Santa Cruz Biotechnology, Heidelberg, Germany) and the HDR-plasmid (#sc-400761-HDR, Santa Cruz Biotechnology) as described (Knorr et al. ). .. The AGS cell line with fak gene knockout was generated using the CRISPR/Cas9 plasmids (#sc-400089-KO-2, Santa Cruz Biotechnology) and the HDR-plasmid (#sc-400089-HDR2, Santa Cruz Biotechnology).

    Knock-Out:

    Article Title: CD4 + T-cells sensitize quasi-mesenchymal breast tumors lacking CD73 to anti-CTLA4 immune checkpoint blockade therapy
    Article Snippet: .. In order to generate the CD73 and B2M double knock out (DKO) cell line, B2M was knocked out from the sgCD73 murine cell line via transient transfection using CRISPR/Cas9 plasmids obtained from Santa Cruz Biotechnology. .. In order to generate the CD73 and B2M double knock out (DKO) cell line, B2M was knocked out from the sgCD73 murine cell line via transient transfection using CRISPR/Cas9 plasmids obtained from Santa Cruz Biotechnology.

    Article Title: Hexokinase-I directly binds to a charged membrane-buried glutamate of mitochondrial VDAC1 and VDAC2.
    Article Snippet: .. Generation of HKI-KO and VDAC-DKO cell lines To knock out HKI in HeLa cells, we obtained a mix of three different CRISPR/Cas9 plasmids for the corresponding gene from Santa Cruz Biotechnology (sc-401753-KO-2). ..



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